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The three elevation gradients that were chosen were 20m, 110m, and 200m. The point centred quarter sampling method was chosen for this study. Transect lines were set up horizontally across each of the three elevation gradients. Five points were set up along the transect lines, five meters apart from one another. As a result, five replicates were set up and randomization was established by placing them five meters apart. A compass was used to make sure that the lines were set up in the same direction. Each of the five points was split up into four quadrants. The distance of the closest black spruce species in each quadrant to the centre point was measured with a measuring tape and recorded. The diameter at breast height (DBH) was also measured for the closest trees by wrapping the measuring tape around the trunk of the tree at 4.5 feet above the ground and then dividing the measurement by 3.14.
I did experience a few problems while implementing my sampling design. I initially went out alone to collect the measurements but then had to return since I realized that I would need help from others to collect the measurements. It was also difficult to walk through the bushes and tree logs to measure the distance. I did notice ancillary patterns in that black spruce species prefer drier conditions as the elevation increases.
