Theoretical Perspective

I have been advancing my research and developing the theoretical framework of my study. For my research paper, I will focus on answering the question as to what factors influence bee pollination of flowers. I will incorporate many ideas from other articles that provide plausible answers to this question. According to the information I gathered from my partial literature review, one example of an aspect that impacts this plant-pollinator relationship is the flower’s UV reflecting colour pattern. This pattern has led to more flower visitation from honey bees compared with a UV absorbing pattern.  Other topics that I will incorporate into my research paper include the “bee avoidance” hypothesis, and the influence of floral signals, such as scent compounds.

Some keywords that I could use to describe my research project include ‘pollination ecology’, ‘plant-pollinator interaction’ and ’floral colour’. These three descriptors really outline the theoretical framework of my study, as to what aspects influence bee pollination of flowers.

Blog Post 6: Data Collection

Throughout this module I needed to get 3 replicate samples completed. I’m collecting a total of 15 replicates (5 from initial data) and either 3 or 4 replicates per module. I haven’t had any problems with implementing my sampling design so far… I will continue to go to my point count stations in the morning when it is nice and cool and I will record the amount of times I see a bird using the area. This no longer takes into account any birds that fly by or and bird calls since they are not technically using the area. I think this will give me more accurate data. So, because of this decision, only part of my data from the first 5 initial observations I recorded is valid and, therefore, I will only be being using half of the data. I will return to all my 5 point count stations in the same order that I did the first time and I’ll continue the process of returning to these stations one at a time over multiple days… This also gives me extra time to complete other parts of this module. So, on Aug 20th I returned to my first point count station. This station is the station with no shelter, this is the lawn area station. It was 15 degrees Celsius, however, there was a layer of smoke blanketing the area. I only saw 1 bird using the area during the whole 10 minutes… I have a feeling this is due to the smoke. I don’t know how long the smoke will stick around and I’m curious if the smoke might skew my observations; however, I can’t wait forever for it to go away and I will just have to take this into account when I am analysing the data. I then returned to my second station a couple days later on Aug 22nd . It was 16 degrees and the thick layer of smoke was still glomming above. The second count station is the intermediate station with half shelter and multiple bird feeders within the area.  Even with the thick smoke I saw 3 birds use the area. The next day, Aug 23rd  ,  I went to visit the third count station. There was no smoke!!! I was a nice morning with clear skies and it was 15 degrees Celsius.  This station is the one right in the middle of the forest with lots of shelter. Here I recorded 3 birds using this station. I will visit the rest of the stations sometime throughout the next couple of days and be finished up by module 8. From the data, I have recorded I am starting to see more of what I was expecting. I did in fact more birds in areas with more shelter than in areas of low to intermediate shelter. I will be very interested when analysing my final data to see if the smoke did in fact have any real affect, but hopefully it will be gone for good now.

Data Collection

I completed my field data collection over the span of two days from 12:00-14:00 at Creekside Park. Throughout the past month, I have been visiting the site and thinking about my research project, notably the hypothesis and study design. My original hypothesis, “the abundance of bees increase around vibrant colourful flowers and decrease surrounding pale white flowers”,  had some subjective terminology that needed clarification. The words ‘vibrant’, ’abundance’ and ‘pale had to either be removed or changed into an objective form. I switched ‘abundance’ with ‘number’, and removed ‘pale’ and ‘vibrant’. I ended up changing my hypothesis to “the number of bees increase around colourful flowers (purple, red, and yellow), and decrease around white flowers”.

I used a random sampling technique to look at  bees around 10 flower samples in the park. I have not experienced many problems with the implementation of my study. The accuracy of my study has improved by implementing my sampling on weekdays, a time frame where their are less people to disturb the bees and flowers.

Post 5: Design Reflections on Leaves

My study design focuses on measuring leaf length of fallen leaves. While this is admittedly easier than climbing trees to collect leaves, fallen leaves bear little loyalty to their parent tree and could blow wherever the wind takes them. Additionally, there is a risk of sampling bias, as more dried leaves take on a dull red colour compared to some of the freshly fallen leaves that have a more vibrant colour. These leaves tend to stand out, which is great but there is some variation in leaf colour, even among freshly fallen leaves. This means those of a duller colour are more likely to be missed. In response to this, I think I will need to use a small quadrat to focus my leaf collection to be exhaustive within a fixed area. That way, all leaves are evaluated to determine if they are freshly fallen enough to be sampled. (Dried leaves are not used because they cannot sustain the manipulation required for measuring and their reduced weight makes them more likely to be dispersed nearby trees by wind from.)

Blog Post #8 Tables and Graphs

Good afternoon Professor Elliot & Class,

It was a challenge to organize and summarize my data, but once I had figured out what the most important parts of my research were, it was easier for me to visualize them. My prediction was that there would be a greater abundance of large woody vegetation (e.g. trees) on the eastern, westerly facing side of Jack Creek.

Hypothesis: If the landscape has a higher elevation, and is westerly facing, then a greater abundance of large woody vegetation will be present.
Prediction: A greater amount of large woody vegetation will be present in western facing slopes with a higher degree of aspect and elevation.
Response Variable: Large woody vegetation abundance
Explanatory (predictor) Variable: Elevation and aspect

During my field data collection, I took counts of both trees and shrubs to create comparison. I took an average of the vegetation count to the number of data points to create an accurate representation of the data. Below are two graphs representing the average number of species in response to elevation and aspect.

Figure. 1 Average number of species is explained in response to changes in elevation. Species are divided into two groups 1. Trees (large woody vegetation) and, 2. Shrubs (small vegetation). Total is included to show linear response over the environmental gradient. In general, total number of species decreased with increases in elevation. The null hypothesis is rejected as there is a greater number of large woody vegetation with increased elevation, however, the greatest abundance of large woody vegetation occurs at an elevation of 540 MASL.
Figure 2. Average number of species is examined in response to varying degrees of aspect (North, North East, East, South East, South, South West, West, North West, and Flat). Species are divided into two groups 1. Trees (large woody vegetation) and, 2. Shrubs (small vegetation). Total is included to show linear response over the environmental gradient. A clear correlation exists between abundance of trees in westerly, south-westerly, and north-easterly facing slopes. This accurately reflects the natural landscape of Jack Creek, which flows north to south within a gully. The null hypothesis is rejected as there is a greater abundance of large woody vegetation on the westerly facing, high elevation slopes.

The outcome of my field studies was slightly different than I expected, however, it reveals that further exploration is necessary as to why there was a greater amount of vegetation in the flat meadows 15 metres on the western side of the creek. Other factors might influence the data, such as disturbance, opens fields, and amount of sunlight.

Blog Post 5: Design Reflections

Initial data collection was successful. Point count locations were selected based on visibility within the drainage channels. Point count locations were also selected based on varying densities of emergent vegetation within the drainage channels in order to provide a representative sample. Point count duration at each point was 5 minutes. Emergent vegetation cover was visually estimated within the visible channel sections. Visibility was greater than 100 metres (m), however, I capped channel section observations to between 60 and 80 metres to increase both the accuracy of waterfowl identification and the overall precision of vegetation estimates.

Prior to my data collection visit on August 17th, I had decided to use a systematic sampling strategy where I overlaid the sitemap with a grid and used a random number generator to select sampling locations. This strategy was unsuccessful due to limited visibility at the randomly selected points. Randomly selected point count locations also did not take into account disturbance of birds within the channels. As the site is diked and exposure to human activity is common, it quickly became important to not only select vantage points that provided adequate visibility but also to select points where waterfowl would be less likely to be disrupted. This modification will improve the accuracy and reliability of my data.

The data collected was generally consistent with my hypothesis in that the total number of waterfowl observed increases with increasing emergent vegetation cover.  I did notice that the total number of waterfowl observed at all point count stations was fairly low. As a result, I will need to ensure that I incorporate additional point count stations and implement enough replicates in order to identify any relevant trends. Initial field data was collected during the hours just before sunset. I intend to collect data just after sunrise in order to determine when waterfowl activity is greatest.

Although the systematic sampling strategy did not pose any major difficulties, I need to consider modifying the strategy slightly to ensure that my replicates are independent of one another.  To ensure independence, I will select channel sections that are at least 100 m apart. This will also reduce the likelihood of counting waterfowl twice. Based on the layout of the survey area, any incoming individuals from nearby point count locations will be highly visible and will not be recorded.

I would also like to be more specific as to what constitutes “emergent vegetation”. Emergent vegetation, for the purposes of this study, will be restricted to yellow pond lily (Nuphar lutea), as it is the most dominant emergent aquatic species within the drainage channels and occurs at varying densities.  This modification will help me to generate a clear and specific research hypothesis and experimental design.

Post 4: Artificial Reality

According to my digital field experiments in the synthesized Snyder-Middleswarth State Park Natural Area, the systematic sampling method was the most time-efficient (12:o6, hh:mm), followed by haphazard (12:23) and random (12:48) methods.

The percentage errors for estimates of the most common and most rare species are shown in the table below:

Percent Error of
Sampling Methods
Species Systematic Random Haphazard
Most Common Species Eastern Hemlock

37%

36%

3%

Sweet Birch

43%

22%

26%

Least Common Species Striped Maple

100%

100%

43%

White Pine

100%

100%

49%

As the data suggest, accuracy of estimates were poorer when the species was more rare. Estimates are more accurate when the species in question occur frequently relative to other species.

Consistently, haphazard sampling produced the sample that most closely reflected true species abundance.

Post 3: Observing Trees and Lichen It

I observed a series of trees along the 1100 block of Meares St. in Victoria, BC. From my observations, I have determined that there are three different species of lichen growing on the bark of the plum trees in this area. Their distributions on the bark of the trees seems consistent with patterns of direct sunlight and shade. Regions of lichen growth inhibition near the bottoms of the trunks are not consistent between trees and follow no discernible pattern of gradient along the street.

 

All of the plum trees seemed to have lost some of their leaves and I wonder if there is a relationship between the number or size of leaves that are being dropped and the position of the tree relative to a nearby construction site and busy roadway. Most of the trees along the street are of similar size and trunk diameter, suggesting they are of similar age. The trees at the west end of the street, near the construction and major roadway are also some of the most heavily-shaded, which may be a confounding variable to any comparisons with this location. The number of leaves dropped by trees in different areas appears fairly equal, but it is hard to say without making measurements if there is a relationship between dropped leaf size and tree.

If there is a difference in the size of dropped leaves, and assuming all trees are of a similar age, this might suggest that trees that are dropping smaller leaves are doing so prematurely, or their leaves are not achieving as large a size at maturity. Either way, the difference might be caused by the growing conditions of the tree (light, water, soil type) or perhaps something is affecting the trees directly. The area of the street near the construction site is noticeably dustier than other areas. We know that trees rely on transpiration through the stomata on their leaves to draw water up from their roots. Perhaps the dust is interfering with this process and leading the trees to drop leaves earlier than normal.

I hypothesize that the size of dropped leaves from trees near the construction site and busy roadway is smaller than the size of dropped leaves from trees at the eastern end of the street. I predict that trees near the construction site will drop leaves of a smaller mean size than trees away from the construction site.

A response variable for this experiment would be the mean length, in millimetres, of leaves (continuous). An explanatory variable would be the distance of the tree from which to leaves are dropped, in meters, to the construction site (continuous).

 

Design Reflections

I conducted my research using a simple random sampling technique to sample how many bees were around different types of flowers. To limit my bias, I used a random number generator on my computer to come up with the number of steps to take (between 1-20 steps) and the compass bearings (between 0-360). I used a .5 meter squared quadrate to analyze how many bees were surrounding the flower of interest. I also used a point count sampling technique to observe how many bees were either on the flowers or an inch away from them for a time period of five minutes. I located 6 flower samples using the random sampling technique and measured the amount of bees surrounding them three times.

I had a couple difficulties implementing my sampling strategy. Some of the coordinates from the random number generator lead me to areas with no flowers. For example, the 3 steps and 213 degree compass bearing led me to the middle of the playground. Another difficulty that interfered with my data collection was disruption from children. Some kids playing around the park would approach the flowers I was observing which may have scarred the bees away. The data I collected did not surprise me in any way because it matched my hypothesis. Overall, I believe that my sampling technique works really well for my research. The one modification I will make to improve my research is to make my observations earlier in the morning to limit the disruption from children.

Blog Post 1: Observation

Location: Riverlot 56, St. Albert, Alberta

Date: August 6, 2018

Time: 1700

Temperature: 29 Celcius

Weather: Sunny with a few clouds

 

Riverlot 56 is located with the city limits of St. Albert on the northeast side.  It is located on Poundmakers road. This particular park is managed by the province as a natural area.  It has several uses including cross-country skiing, snowshoeing, hiking and wildlife viewing.

The total size of the park is 266.86 Acres (108 Hectares).  

The area that was chosen is an open field in the park that is surrounded by trees on three sides.  On the fourth side is a fence with farmland on the other side of this fence.

There are many gopher holes that can be seen from the pathway.  

This land is mostly made up of tall and short grasses with Canada Thistle scattered throughout.  On initial appearances it appears to be a healthy natural grassland area. However, on closer inspection there appears to be large areas of thistle.

Potential Subjects:

  • Amount of Canada thistle present compared to other plants
  • Number of gopher holes and the effect they have on the surrounding grassland
  • There will be more invasive plants closer to the trails than the untouched pasture
  • pH in the soil affects what will grow

 

Overall facing South
Overall Facing East
Overall Facing NorthEast

Riverlot 56 sketch 1