Blog Post 7 for Katarina Duke

Theoretical basis of my research project is to demonstrate how algae growth varies depending on the flow regime of freshwater creeks. The growth of algae can act as an indicator for water quality (i.e. water pollution) and as a predictor for the maintenance of water supply systems (i.e. intake pipes and filter lines). Algae can deplete the oxygen in water, release toxins, and lead to taste and odour issues. More turbulence leads to more oxygen absorbed by water, thus counteracting the oxygen depleted by algae. The creeks sampled in my research project have proven to be fish bearing through previously conducted fish presence studies and observation. Establishing the connection between algae growth and flow regimes within freshwater creeks will aid in maintaining a healthy ecosystem for fish and predict creeks potentially at risk for loss of fish.

Ecological processes that my hypothesis will touch on are the hydrologic cycle and nutrient cycling.

It is also important to acknowledge that temperature, seasonality, weather, and unknown anthropogenic activities can affect the growth rate of algae. Other studies have been completed focusing on the relationship between nutrient levels and algae growth as well as temperature and algae growth.

Keywords: Algae growth, flow regime, water depth, turbulent

Blog Post 6 for Katarina Duke

On average 20-25 flow measurements were taken within each creek to determine the flow discharge with 5 discharge measurements obtained per creek for a total of 25 discharge measurements. No issues occurred in implementing the sample design due to the resources available; the SonoTek Flow Tracker 2 helps eliminates sampling issues by providing quality control errors for flow measurements taken within the creek to help prevent flow measurement errors form compounding onto the discharge measurement.

Preparation for data collection was minimal as the only supplies needed in the field was a journal, SonoTek Flow Tracker 2, and spare batteries. I was not required to make tables prior to arriving in the field as the SonoTek Flow Tracker 2 stores all data that can then be downloaded onto a computer.

It has been observed that despite all the creeks being hydraulically connected to the Miracle Valley aquifer the drops in flow rate into low season of each of the creeks did not happen at the same rate. This allows me to infer that the creeks are hydraulically connected to the aquifer to varying degrees.
I also observed the presence of a fish farm in the area, west of Belcharton Creek before its confluence with Lagace Creek This did raise concerns that the algal growth observed within the creeks could be a result of excess nutrients from the farms; however, algal growth was more prominent in Seux Brook that is not within proximity of potential discharge from the fish farm. Also, since no algal growth was observed in Lagace Creek my concerns were absolved since Belcharton is a tributary of Lagace Creek.

Blog Post 5 for Katarina Duke

The only real challenge that presented itself was finding a viable location within each creek to perform discharge measurements. The device used for obtaining the discharge measurements requires a fairly cobble-free creek bed, no obstructions to flow within the creek (i.e. logs), and a visually uniform flow (i.e. avoid switchbacks). This proved to be challenge for Durieu Creek and Legace Creek as they experience a great deal of sediment deposition. Durieu Creek also follows a steep terrain with many switchbacks causing many sections of the creek to be unsuitable for obtaining measurements. Selection of the creeks for the study was chosen systematically based on their hydraulic connection with the Miracle Valley aquifer. Locations for sampling along the creeks were conducted using random sample selection. I intend to collect data using the same technique as it is the method defined in the Manual of British Columbia Hydrometric Standards and Environment Canada’s “A Field Guide to Streamflow Measurement by Gauging and Metering.” I will also continue to use the SonoTek Flow Tracker 2 as it is widely used by water resource professionals and provides quality control warning messages to assist with data accuracy. The location of measurements will not be changed as a base point is required to determine changes in water depth. Also differences in creek bed composition (i.e. amount of cobbles) can affect flow measurements. By using the same locations and equipment the influence of confounding variables can be controlled.

 

 

Response post was completed for:

Blog Post 3: Ongoing Field Observations

Blog Post 3 for Katarina Duke

I intend to study the relationship between abiotic factors and algal growth in freshwater streams within the Miracle Valley. The study will be focused on the impact water depth and flow regime have on algal growth.

Five creek locations within the Miracle Valley were sampled on May 31, 2018 and June 1, 2018 for flow discharge rates and water depth. The results of the measurements are shown below in the table below.

Additional observations from the site visit include are as shown in pages from field book.

July 5 Field Notes Page 1 July 5 Field Notes Page 2 June 1 Field Notes June 19 Field Notes Page 1 June 19 Field Notes Page 2 May 15 Field Notes Page 1 May 15 Field Notes Page 2 May 15 Field Notes page 3 May 31 Field Notes

I hypothesize that a relationship exists between algal growth and flow regime and water depths. I predict that as water depths decrease, provided creeks are not intermittent (i.e. run dry depending on seasonality) algal growth will increase and turbulent waters will reduce algal growth.

The hypothesis will be tested using a SonoTek Flow Tracker 2 to measure flow discharge rates at same location each site visit.  Rebar will be used as a staff gauge to measure difference in water level before and after discharge measurements and to compare with measurements taken on other days. A scale of none-to-moderate-to significant will be used to estimate algal growth.

The experiment will be conducted using logistic regression and the hypothesis is that slower flow regimes and shallow water depths increase algal growth.

In this experiment the response variable is algal growth that is categorical (using a scale of none, minimal, moderate, significant, very significant). The explanatory variables are continuous and include the flow rate and water depth.

Blog Post 1 for Katarina Duke

The study location is known as Miracle Valley, sometimes also referred to as Upper Hatzic Valley that extends from Lagace Creek at the south to the Stave Lake reservoir at the north. The valley is bounded by steep mountainous terrain to the east and west.

Land use in the area is predominantly forest, accompanied by rural residential and low-intensity agricultural uses. Ground elevations within the area rise abruptly north of Durieu Road and then plateau and begin to decline at Stave Lake.

As per data from the nearest climate monitoring station—courtesy of Environment Canada—the area receives approximately 1,808mm of precipitation annually with approximately 68% of the total annual precipitation falls between the months of October and March (Government of Canada, 2018).

The watercourses included in the study include Lagace Creek that crosses the south end of the Valley that obtains flows from tributaries to the west. (i.e. Belcharton Creek, Durieu Creek, Oru Creek, and Seux Brook). The tributary creeks are incised into steep-sided ravines and are declared to be largely spring-fed, due to relatively constant flows throughout the year (Piteau Associates Engineering Ltd., 2012).

A reconnaissance site visit was completed on May 15, 2018 from 08:00 to 15:35. The weather was predominantly sunny with about 20% cloud cover and a temperature ranging from approximately 9 to 21 degrees Celsius throughout the day.

Map of Sampling Locations

Observations:

  • Note: Groundwater fed creeks
  • algal growth observed in Seux Brook in abundance prior to confluence of Seux Brook and Oru Creek
  • No algal growth observed in Legace Creek, Belcharton Creek, Oru Creek or Durieu Creek
  • No moss present on trees within immediate proximity of Legace Creek
  • Significant stream bank vegetation growth observed on Belcharton Creek
  • Heavy amounts of moss present on trees surrounding Durieu Creek
  • Clusters of moss present on trees surrounding Belcharton Creek
  • Variety of avian species and squirrels observed around Oru Creek
  • Water seepage observed in area around Durieu Creek.
  • Grasses growing in and along stream bank of Seux Brook
  • Flow discharges (highest to lowest) Legace, Belcharton, Oru, Durieu, Seux
  • Durieu Creek and Legace Creek bed heavily cobbled, turbulent flows
  • Seux Brook and Oru Creek observed relatively laminar flows
  • Seux Brook bed silt and clay
  • Seux Brook is partially shaded prior to confluence with Oru Creek
  • Oru Creek is not shaded at all
  • Durieu Creek is heavily shaded
  • Belcharton and Legace Creek are shaded in sections throughout water course

May 15 Field Notes Page 1 May 15 Field Notes Page 2 May 15 Field Notes page 3

Questions that could be proposed from the observation is:

  1. Is the location of moss growth on trees an accurate way to determine cardinal direction?
  2. Does water depth influence algal/vegetation growth?
  3. Does flow regime influence algal/vegetation growth?
  4. Does the extent of cobbles within creek beds influence algal/vegetation growth?
  5. How does the weather (i.e. sunlight and temperature) influence algal/vegetation growth?
  6. Does the flow regime and water depth change due to seasonality and weather?

PHOTOS:

Katarina Duke- May 15 Photos

REFERENCES:

Government of Canada. (2018, April 24). Station Results – Historical Data. Retrieved from Government of Canada: http://climate.weather.gc.ca/historical_data/search_historic_data_stations_e.html?StationID=702&Day=20&timeframe=1&type=line&MeasTypeID=dptemp&Month=7&Year=2016&searchType=stnProx&txtRadius=25&optProxType=navLink&txtLatDecDeg=49.025277777778&txtLongDecDeg

Piteau Associates Engineering Ltd. (2012). MISSION HYDROGEOLOGICAL INVESTIGATION FOR GROUNDWATER SUPPLY MIRACLE VALLEY, B.C. North Vancouver: Piteau Associates Engineering Ltd.

 

Blog Post 4 for Katarina Duke

Three sampling methods were used in gathering data from the Mohn Mill community using the virtual forest tutorial: haphazard, random, and systematic.

An equal number of quadrats were sampled (i.e. 30 each) with the systematic sampling technique having the fastest sampling time but, the sampling time for all three methods remained within the range of 15 to 16 hours. The haphazard method had a sampling time of 15 hours and 57 minutes, and the random sampling method has a sample time of 15 hours and 49 minutes.

In all three sampling strategies, Red Maple and White Oak were determined to be the two most common species; However, the results for the two rarest species differed for each method (i.e. American Basswood, Sweet Birch, White Ash, and Hawthorn).

  1. Haphazard or convenience sampling

Using the area, haphazard sampling technique for the Mohn Mill community, American basswood and Hawthorn were the two rarest species as indicated by the actual importance value.

·         Hawthorn

Actual importance value: 0.6

Calculated importance value: 0.4

 

·         Sweet birch

Actual importance value:  0.2

Calculated importance value: 0.7

 

 

  1. Random sampling

 

Using the area, random sampling technique for the Mohn Mill community, White ash and Hawthorn were the two rarest species as indicated by the actual importance value.

 

·         White Ash

Actual importance value: 0.2

Calculated importance value: 0.6

 

·         Hawthorn

Actual importance value: 0.6

Calculated importance value: 0.6

 

 

 

 

  1. Systematic sampling

 

Using the area, systematic sampling technique for the Mohn Mill community, American basswood and Sweet birch were the two rarest species as indicated by the actual importance value.

·         American basswood:

Actual importance value: 0.2

Calculated importance value: 1.5

 

·         Sweet birch:

Actual importance value: 0.2

Calculated importance value: 0.7

 

For all three sampling methods—haphazard, random, and systematic sampling—the accuracy improved with abundance.

Of the three methods, the random sampling method had the highest accuracy.

I found it interesting that the systematic method of sampling had skewed the density of the rare species to such a substantial extent, making a haphazard sampling approach appear to be a more desirable sampling method. I was also surprised to see haphazard having the degree of accuracy it did.

A reason for the lack of accuracy using the systematic sampling method could potentially be using a transect sampling method in conjunction with the systematic method. I selected the samples at regular distances along the transect, with the initial point randomly chosen. As stated in “Tutorial: Sampling techniques,” systematic sampling can produce problems if the points correspond to an underlying environmental pattern, which perhaps is the case for Mohn Mill community.

I am curious about the results stratified sampling and transects would obtain. For stratified sampling, the tree population would be split into somewhat homogenous groups (same species). I predict the accuracy for stratified sampling would be equivalent to, if not better than, the accuracy of random sampling and that the common species determined would match. I think stratified sampling would determine the rarest species to be Hawthorn and Sweet birch due to their occurrence in two of sampling methods used.

A method I am aware of that is commonly used in the forestry industry is the point-centered quarter method, where a point in the center of the forest is identified and then the area surrounding it is separated into four quarters. I am surprised this method was not within the tutorial given its common use in relation to trees. I’d be interested in seeing how the method compares to those used within the tutorial in terms of the rare species determined and accuracy.

 

Blog Post 2 for Katarina Duke

A source of ecological information is the International Journal of Ecology which can be found by clicking the URL link provided below.

https://www.hindawi.com/journals/ijecol/

A peer-reviewed research article requires the works to be written by an expert in the field, include in-text citation, contain a bibliography, be reviewed by a referee prior to publication, and report methods and results of a field study.

An article that interested me and meets the requirements to be classified as a peer-reviewed research article is “Benthic Macroinvertebrates Diversity as Bioindicator of Water Quality of Some Rivers in East Kalimantan, Indonesia.” (Refer to attachment for research article)

The objective of the research was to clarify and evaluate the water quality of several rivers in East Kalimantan province of Indonesia by utilizing the benthic macroinvertebrates diversity and physical-chemical parameters of the river water.

The research article was written by Fatmawati Patang, Agoes Soegianto, and Sucipto Hariyanto belonging to the Department of Biology, Faculty of Sciences and Technology at the Universitas Airlangga. All experts in the field of water quality, biology, and toxicology. For instance, Agoes Soegianto is a PhD recipient and professor at Universitas Airlangga. He is an expert in marine biology, environmental contamination, and ecotoxicology, having completed over 40 research works in his field.

The article includes in-text citation accompanied by a bibliography and was reviewed by Panos V. Petrakis prior to publication. Petrakis is associated with the Institute for Mediterranean Forest Ecosystems as a research scientist. Petrakis’ expertise in ecology and biodiversity makes him a suitable candidate for referee for the research article.

The source also reports the result of a field study completed by the authors who include in the article their method and results. Their method included sampling a minimum of 100 individual benthic macroinvertebrates and the measurement of numerous water quality parameters (i.e. dissolved oxygen, biological oxygen demand, pH, turbidity, etc.). The macroinvertebrates were identified and enumerated to calculate the diversity, dominance and evenness index as they are frequently used to predict the conditions of aquatic environments. The results supported their hypothesis that the Karang Mumus River recently received pollutants that could be classified as dangerous.

To further support the classification of the article as peer-reviewed research material, the article is offered though the International Journal of Ecology that operates as a peer-reviewed open-access journal.

Blog 4: Sampling Strategies

The virtual forest tutorial had three different strategies to collect data which are systematic sampling, random sampling, and haphazard sampling. The strategy that had the quickest estimated time was systematic sampling with a time of 12 hours and 37 minutes. Not far behind though was random sampling at 12 hours and 45 minutes, and in last with the slowest time of 12 hours and 58 minutes was haphazard sampling.

The 2 most common tree species were the Sweet Birch and the Eastern Hemlock, and the systematic sampling technique proved to be the most accurate for them. Random sampling also did okay and so did haphazard, but that was only for the Sweet Birch, whereas for the Eastern Hemlock the error was much higher.

Systematic 

E. Hemlock – 17.4% error,  S. Birch- 15.7% error

Random

E. Hemlock- 21% error, S. Birch- 26.2% error

Haphazard

E. Hemlock- 47.4% error, S. Birch 22.3% error

When it came to the two least common species, White Pine and Striped Maple, the technique that seemed to be most accurate overall was random sampling, with an exception being 15.1% error using the haphazard method for Striped Maple.

Systematic

W. Pine- 131.7% error, S. Maple- 129.9%

Random

W. Pine- 49% error, S. Maple- 17.4%

Haphazard 

W. Pine- 175.8% error, S. Maple- 15.1%

I found that was the abundance of the species decreased, the percentage error increased.

Blog Post 4: Sampling Strategies (Percy)

The technique that had the fastest estimated sampling time:

Random/systematic sampling of area (12 hours, 33 minutes) as opposed to 12 hours, 36 minutes and 12 hours, 40 minutes for the other sampling techniques.

Percent error:

Most common species include the Eastern Hemlock; Random/Systematic 14.25%, Haphazard 9.52%

Sweet Birch; Random/Systematic 24.68%, Haphazard 20.83%

Most rare species include the Striped Maple; As these were predicted to not be present in these samples, the percent error is negative.

White Pine; (above). The accuracy of this data corresponds to the amount of species within the given area, as the more species, the more accurate the results. The sampling strategy that seemed most appropriate for this experiment would be Systematic sampling of a given area as it was much more accurate and time-efficient.

Blog 3: Ongoing Field Observations

I returned this morning, June 26, to McArther Island to observe the main pond in the golf course and the moat surround in the island. The three gradients I have chosen are the main pond because it is the largest of the three on the course, a section of the moat right by the bridge, and another section of the moat that is at the other end the the island near the boat launch closer to the mouth of the river. I have decided to specifically study the mallard duck (Anas platyrhynchos) and their habitat preferences. I would like to know why I have observed so many ducks around the bridge in the moat rather than in the quieter area of the pond or nearer to the opening of the river into the moat. I hypothesize that the mallard duck (Anas platyrhynchos) prefer the bridge area of the moat. I predict this is because the water is of better quality, there is a greater food source and also because there is more shade during the day due to overhanging trees and shrubs. My predictor variables are: water alkalinity, pH, calcium and chlorine, water temperature, types of food source, shade vs sun, and these variables are categorical. The response variable is the number of mallard ducks present which is a continuous variable. This informations shows me that my experiment will be an ANOVA multi factor design.

Here are my three different gradients:

1.Golf course main pond

2. Moat by bridge

3. Moat by river